Figure 1.

Celastrol (CLT) reduces renal lesions and improves renal function in IgAN mice. (A) Schematic diagram of IgAN mice model construction and CLT treatment process. (B) Representative images of glomerular Immunofluorescence staining with IgA (Bars=50μm). (C) The average optical density of IgA was analyzed using ImageJ software. (D) Representative micrographs following periodic acid-Schiff staining (Bars=50μm). Histopathology scoring of (E) glomerular proliferation and (F) sclerosis was based on an evaluation of 80 glomeruli per group (20 glomeruli per animal, 80 glomeruli in total) and is reported as % glomeruli proliferation or sclerosis. The effect of CLT on (G) serum creatinine (SCr) and (H) urinary protein/creatinine ratio (uPCR) in IgAN mice; Statistical differences in multiple groups were determined by one-way ANOVA followed by Tukey's multiple comparisons. All data is represented as mean ± SD, n=4 for each group. Images were captured at ×400 magnification. **: p < 0.01, ***: p < 0.001, vs. CON. #: p < 0.05, ###: p < 0.001, vs. IgAN. ns: p > 0.05.