Figure 6.

CLT inhibited macrophage M1-like polarization via the ECM1/STAT5 pathway. (A) Representative images of STAT5 and p-STAT5 immunofluorescence staining in each group of mice kidneys (Bars=50μm). (B) The relative average fluorescence intensity of p-STAT5/STAT5 was analyzed by ImageJ software.(C) Representative western blots and (D) quantitative analysis of STAT5 and p-STAT5 in LPS-induced Raw 264.7 cells treated with CLT. (E) Representative western blots and (F-G) quantitative analysis of ECM1, STAT5, and p-STAT5 in ECM1-overexpression Raw 264.7 cells. Statistical differences in multiple groups were determined by one-way ANOVA followed by Tukey's multiple comparisons. All data were presented as mean ± SD, n=4 for each group of mice, n=3 for each group of cells. #: p < 0.05, ##: p < 0.01, vs. LPS. ^p < 0.05, ^^p < 0.01 vs. LPS-CLT-NC. ns: p > 0.05.