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. 2024 Oct 7;20(14):5415–5435. doi: 10.7150/ijbs.97669

Figure 7.

Figure 7

HB promotes autophagy by increasing TFEB expression levels after SCI. (A) Representative images of immunofluorescence staining for TFEB (green) and NeuN (red) in the injured spinal cord ventral horn grey matter on day 3 after SCI. (B-C) The illustration showing the quantitative results indicates that HB increased the integrated density of TFEB in spinal cord neurons and the integrated density of TFEB in the nucleus of each neuron. (D-E) WB analysis of cytoplasmic and nuclear TFEB levels in the injured spinal cord lesions on day 3 after SCI in each group (Sham, SCI, SCI + HB, SCI + HB/scrambled shRNA, and SCI + HB/TFEB shRNA groups). The data were normalized to β-actin or histone H3. (F-G) Typical immunofluorescence staining images of spinal cord ventral horn grey matter on day 3 after SCI, with the number of LC3 II-positive puncta in neurons shown in the graph; scale bar: 20 μm. (H-J) WB analysis of VPS34, SQSTM1, Beclin1, CTSD, LC3, NLRP3, caspase-1, IL-1β, GSDMD-N, IL-18 and ASC levels in injured spinal cord lesions on day 3 after SCI. GAPDH was utilized as a loading control. Densitometry quantification of the expression of autophagy- and pyroptosis-related proteins in the injured spinal cord lesions. The data are presented as the means ± SEM (n = 6 mice per group); *P < 0.05, **P < 0.01.