Figure 6.

TN41 induced Activation of the MAPK pathway in DPCs. (a) IF images of CD34 (HFSC marker) and LEF1 in hair follicles from TN41-treated and vehicle-treated mice. Relative LEF1 FI was measured in the DPC. (b) IF images of Ki67 and c-Myc in the hair follicle. Ki67-positive cell was counted and the relative FI of c-Myc was measured in the DPC. (c and e, f) DPCs were treated with time and dose-dependent TN41, and WB were performed via each antibody. (d) DPCs were treated with 5.1 nM of Wnt3a, 8.9 nM of FGF7, and 20 nM of TN41 for 14 h, and relative gene expression levels were measured by quantitative PCR. (g) Regulatory effect of TN41 on the expression of various growth factors in DPCs. The bars: 20 μm (a and b). Error bars: mean ±SD. ***: P < 0.001, **: P < 0.01, *: P < 0.05.