Figure 7.

Effects of TN41 on human DPCs. (a) Effects of TN41 in human hair shaft elongation. Human HFs were cultured for 6 days with TN41, or the vehicle and hair length were measured (3 donors). (b) IF image of Ki67 in human hair follicles and relative FI was measured by Image J. (c) 3D-cultured DPCs with or without TN41 were injected into the hypodermis of nude mice. Images were obtained after 2 weeks (injection site = 3) and measured neogenesis HF numbers. (d) Gene categories enriched in TN41-treated DPCs measured by RNA sequence. Extracellular signaling molecules were among the most enriched (blue box). (e) List of upregulated DPC genes in the extracellular signaling category. (f) TN41 treatment induced Wnt and FGF pathway genes in DPC. The microarray signal values of Wnt and FGF pathway members displayed temporal differences. (g) RNA-seq heatmap depiction of 42 DPC genes upregulated by TN41 in DPCs co-cultured with HFSCs and treated with vesicles and Wnt3a (shown in Fig. 7e). The fold changes (FC) were shown by color gradient. Blue box: positive genes, red box: negative genes. Error bars: mean ± SD. ***: P < 0.001, *: P < 0.05.