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Expression of the Rad51C protein is restored in complementing CL-V4B/#17 and CL-V4B/hRad51C cells. V79B, CL-V4B, CL-V4B/hRad51C, CL-V4B/#17 (hybrids 1, 4, 5) and human immortal fibroblast MRC5V1 cells were lysed and whole cell extracts were electrophoresed on a 12% SDS–PAGE gel and transferred to a membrane. The Rad51C protein was visualized by using polyclonal rabbit anti-hRad51C antibodies and actin by using mouse anti-actin antibodies to confirm equality of loading.
