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. Author manuscript; available in PMC: 2024 Nov 1.
Published in final edited form as: Clin Infect Dis. 2012 Mar 12;54(8):1187–1195. doi: 10.1093/cid/cis015

Table 3.

Mutation Profiles of Plasma and DBS Specimens

Plasma Specimens
 DBS Specimens
Specimen Type Specimen ID ARV Regimen PI NRTI NNRTI PI NRTI NNRTI
Matched specimens
Concordant
 ≥1000 copies/mL 2-2-063 AZT, 3TC, NVP K101E, E138A, G190A K101E, E138A, G190A
1-2-095 3TC, d4T, NVP T69ST T69ST
2-2-028 AZT, 3TC, NVP G190A G190A
2-2-132 3TC, TDF, NVP K65R, M184V Y181C K65R, M184V Y181C
1-2-128 3TC/AZT, NVP L10I V75IV, K219EK Y181C L10I V75IV, K219EK Y181C
2-2-010 AZT, 3TC, NVP K70R, M184V A98AG, Y188L K70KR, M184V A98AG, Y188L
2-2-041 FTC/TDF, NVP K65R, M184V L100I, K103N K65R, M184V L100I, K103N
1-2-176 3TC, d4T, NVP
1-2-121 3TC, d4T, EFV L10I L10I
2-2-118 3TC, d4T, EFV V118I, M184V K103N, P225H V118I, M184MV K103 KN, P225HP
1-2-030 3TC, d4T, NVP L10IV L10IV
1-2-016 3TC/AZT, NVP T74S K103KN T74S K103KN
1-2-186 Unknown T74S D67G, K70R, M184V, K219E A98G, V108I, Y181C T74S D67G, K70R, M184V, K219E A98G, V108I, Y181C
1-2-102 3TC, d4T, NVP M184V K103N, K238EK M184V K103N, K238E
 <1000 copies/mL 2-2-091 AZT, 3TC, NVP M184V K101E, E138A, G190A, M230L M184V K101E, E138A, G190A, M230L
Discordant
 ≥1000 copies/mL 2-2-120 a FTC/TDF, NVP D67DN, K70KR, M184V, K219DEKN A98AG, V179DE, Y181I K65R, D67N, K70KR, M184V, K219E V179DE, Y181I
2-2-105 AZT, 3TC, NVP L10I M184MV K103N L10I M184MV K103N, H221HY
1-2-100 3TC, d4T, NVP L10I M184V A98AG, Y181C, H221HY L10I M184V Y181C, H221Y
2-2-128 FTC/TDF, NVP M184V A98G, K101KQ, V108IV, Y181C, H221HY M184V A98G, V108I, Y181C
2-2-116 a FTC/TDF, NVP L10I M184MV A98G, Y181C, H221Y L10I A98G, Y181CY, H221Y
 <1000 copies/mL 1-2-099 a 3TC, d4T, NVP M184V K103N, V108IV
2-2-130 FTC/TDF, NVP A62AV, K65KR, M184V, K219KN K103N, Y181C L74V, M184V K103N, Y181C
Unmatched specimens
 <1000 copies/mL 2-2-053 a AZT, 3TC, NVP D67DN, K70KR, M184V, T215FIST Y181C, H221HY NDb NDb NDb
1-2-167 3TC/AZT, EFV M46L NDb NDb NDb
2-2-060 FTC/TDF, EFV No Mutations NDb NDb NDb

Mutation profiles from all plasma specimens that were genotyped are shown, along with the corresponding dried blood spot (DBS) specimen. Matched refers to specimens in which both the plasma and DBS specimens were genotyped; unmatched, specimens in which we were able to genotype only the plasma specimen owing to poor polymerase chain reaction (PCR) amplification of the DBS specimen. Boldface indicates mutations that were discordant between plasma and DBS specimens.

Abbreviations: 3TC, lamivudine; ARV, antiretroviral; AZT, zidovudine; d4T, stavudine; EFV, efavirenz; FTC, emtricitabine; ND, not determined; NRTI, nucleoside reverse-transcription inhibitor; NNRTI, non-NRTI; NVP, nevirapine; PI, protease inhibitor; TDF, tenofovir disoproxil fumarate.

a

Specimen pairs for which plasma and DBS specimens had differing drug susceptibility (susceptible, intermediate level, or high level resistance) ratings, as determined by the Stanford University HIV Drug Resistance Database HIVdb algorithm.

b

Not determined due to RT-PCR was negative.