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. 2002 May 15;30(10):2144–2153. doi: 10.1093/nar/30.10.2144

Figure 2.

Figure 2

Characteristics of DNA gyrase decatenation activity. (A) Mycobacterium smegmatis holoenzyme is required for decatenation activity. The reaction, containing 0.5 µg kinetoplast DNA with individual subunits alone (lanes 2 and 3) or together (lanes 4–8), was carried out in the presence of 10 µg ml–1 ciprofloxacin (lane 5), 20 µg ml–1 novobiocin (lane 6) and 20 µg ml–1 each of GyrA-specific mAbs C3 (lane 7) and E9 (lane 8). (B) The decatenation reaction was carried out with M.smegmatis (2 U) and E.coli (4 U) DNA gyrases. M, 1 kb DNA ladder; kN, kDNA network; BN, broken network. (C) Quantitative analysis of minicircles released with various concentrations of E.coli and M.smegmatis enzyme. (D and E) Quantitative analysis of the effect of Mg2+ concentration on DNA supercoiling and decatenation activities of E.coli and M.smegmatis enzymes.