Figure 3.

Native and denaturing acid gels of aminoacylated minimalist tRNA^leu-ΔACVL molecules. Each aminoacylation reaction was performed with either 10 or 60 µM RNA, 20.5 µM ¹⁴C-leucine (50 µCi/ml) and 100 nM LeuRS under the conditions described in Materials and Methods. (A) RNA bands were detected by ethidium bromide staining of a native acidic gel. (B) RNA aminoacylated with ¹⁴C-leucine was visualized by phosphoroimaging the dried acidic gel for two weeks. Arrows in both (A) and (B) emphasize four distinct RNA bands observed using native acid gel electrophoresis. (C) Denatured RNA bands were detected by ethidium bromide staining of a denaturing acidic polyacrylamide gel. (D) Denatured ¹⁴C-leucine aminoacylated RNA products were visualized by phosphoroimaging the dried acidic gel for two weeks.