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Hydrolytic amino-acid editing for each of the minimalist variants aminoacylated with 3H-leucine. All reactions contained ∼18 µM aminoacylated substrate, 100 nM of either wild-type LeuRS or T252A mutant enzyme, 60 mM Tris (pH 7.0) and 10 mM MgCl2. Editing activity was tested for tRNAleu-ΔVL (A) and tRNAleu-ΔAC (B).
