Table 2. Kinetic analysis of minimalist tRNAleu substrate leucylation by E.coli LeuRS.
| tRNAleu minimalist substrates | KM(µM) | kcat(min–1) | kcat/KM(µM–1 min–1) | kcat/KM (relative) | Loss |
|---|---|---|---|---|---|
| tRNAleu (UAA) | 0.9 | 0.354 | 3.9 × 10–1 | 1 | 0 |
| tRNAleu -ΔAC | 7.8 | 0.188 | 2.4 × 10–2 | 0.06 | 17 |
| tRNAleu-ΔVL | 23.5 | 0.071 | 3.0 × 10–3 | 0.008 | 125 |
| tRNAleu-ΔACVL1 | NM | 1.56 × 10–3 | NM | NM | NM |
| tRNAleu-ΔACVL2 | NM | 8.80 × 10–3 | NM | NM | NM |
| tRNAleu-ΔACVL3 | NM | 7.45 × 10–3 | NM | NM | NM |
| tRNAleu-ΔACDL4 | - | - | - | - | - |
| tRNAleu-ΔACDL5 | - | - | - | - | - |
| tRNAleu-ΔACDL6 | - | - | - | - | - |
| tRNAleu-ΔACDL7 | - | - | - | - | - |
| Minileu3B | - | - | - | - | - |
NM, aminoacylation occurs but kinetic parameters are not measurable.