Table 1.
Gene-specific primers and annealing temperatures used for quantitative real-time PCR analysis.
| Gene name | Primer (5’-3’) | Annealing temperature (°C) | |
|---|---|---|---|
| Muc2 | F | GAACGGGGCCATGGTCAGCA | 60 |
| R | CATAATTGGTCTGCATGCC | ||
| Reg3γ | F | CCTCAGGACATCTTGTGTCTGTGCTC | 68 |
| R | TCCACCTCTGTTGGGTTCATAGCC | ||
| Dmbt1 | F | GGGGATCTCCACTGTTATCTTTGA | 60 |
| R | AGAATCTGTTCCATCTGTGGGA | ||
| mLys | F | GGCTGGCTACTATGGAGTCAGCCTG | 65 |
| R | GCATTCACAGCTCTTGGGGTTTTG | ||
| mBD4 | F | CCACTTGCAGCCTTTACCC | 63.3 |
| R | GCCAATCTGTCGAAAAGCGG | ||
| Lypd8 | F | GCCTTCACTGTCCATCTATTT | 60 |
| R | GTGACCATAGCAAGACATGCA | ||
| ZO-1 | F | CCACCTCTGTCCAGCTCTTC | 55 |
| R | CACCGGAGTGATGGTTTTCT | ||
| Claudin-1 | F | TCCTTGCTGAATCTGAACA | 53 |
| R | AGCCATCCACATCTTCTG | ||
| TNF-α | F | ACGGCATGGATCTCAAAGAC | 55 |
| R | AGATAGCAAATCGGCTGACG | ||
| IL-1β | F | AACCTGCTGGTGTGTGACGTTC | 55 |
| R | CAGCACGAGGCTTTTTTGTTGT |