Figure 7.
Investigating outer membrane stability of strains. Panel a shows growth on LB agar plates (LB) compared with growth on plates additionally containing 2% of the envelope stressor sodium dodecyl sulfate (SDS); 5 μL of 10−3 −10−7 dilutions of overnight cultures were spotted in replicates (n = 2) on plates and incubated at 37°C under anaerobic conditions. Below (panel b) displays results challenging strains with SDS (0.025%) followed by recording the percentage of SYTOX green positive cells by flow cytometry.