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. 2024 Nov 1;10(44):eado6765. doi: 10.1126/sciadv.ado6765

Fig. 5. COX20 is downstream regulated by PSIP1.

Fig. 5.

(A) Enrichment for AF4 and ENL targets (ChEA2022) in the genes identified in CUT&RUN analysis for PSIP1 binding in Jurkat. (B) Heatmap representing the top 50 differentially expressed genes upon PSIP1 knockdown in Jurkat (n = 5, time: 72 hours). (C) GSEA enrichment plot shows a negative enrichment for the assembly of cytochrome c oxidase upon knockdown of PSIP1 expression in Jurkat. FDR, false discovery rate; GOBP, Gene ontology biological process; NES, normalized enrichment score. (D) Normalized counts for COX20 from RNA-seq data that were described in (B). COX20 is severely down-regulated upon down-regulation of PSIP1 expression. (E) RT-qPCR for PSIP1 and COX20 upon PSIP1 knockdown in Karpas-45 and Jurkat cells (n = 3, one-way ANOVA with Dunnett’s multiple comparisons test). (F) Western blot validation of COX20 down-regulation on protein level in Jurkat (one blot representative for three replicates, normalization: n = 3, one-way ANOVA with Dunnett’s multiple comparisons test). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.