Fig. 5. Positive and negative regulations of antitumoral immune responses come together in the T/DC community.

(A) Minimum distance of DCs and CD103⁺ DCs that have “low” or “high” CXCL9 expression (threshold = 0.5) to PD-1⁺ CD8⁺ T cells within 800 pixels in T/DC community from dataset 2. Distance values were log₂ scaled. ***P < 0.001. (B) Visualization of cell outlines for cells assigned to T/DC community in MRTX1257-treated tissues from dataset 2, with CXCL9-high DCs, CD103⁺ DCs, and PD-1⁺ CD8⁺ T cells colored in to show spatial proximity of these cell phenotypes. Some regions were expanded for easier visualization. (C) Mean expression of Ki67 on CD4⁺ and CD8⁺ T cells and T_regs within the T/DC community for vehicle and MRTX1257 treatment groups from dataset 2. Values were log₂ scaled. (D) The number of times a c-casp3⁺ tumor cell is found in the 15-pixel neighborhood of a CD8⁺ T cell within the T/DC community, compared across vehicle and MRTX1257 treatment groups for dataset 2, averaged per ROI. Count is relative to the proportion of tumor cells that were c-casp3⁺ in vehicle versus MRTX1257 treatment groups. Each dot represents the value of one ROI. (E) Log₂ fold changes (log₂FC) in enrichment from neighbouRhood analysis for CD8⁺ T cells in the T/DC community following treatment with MRTX1257. Filled circles represent images from which enrichment was statistically significant compared to randomized spatial arrangements following treatment with MRTX1257 for dataset 2. (F) Scaled proportion of T_regs contributing to each of the 18 original communities for dataset 2. (G) Visualization of cell outlines for cells assigned to the T/DC community in MRTX1257-treated tissues for dataset 2. DCs, CD103⁺ DCs, CD4⁺ and CD8⁺ T cells, and T_regs were filled in to illustrate spatial proximity of these cell types. Some regions were expanded for easier visualization. MRTX, MRTX1257.