Fig. 1 ∣. Cryo-EM structure of TTLL6 bound to the MT.

a, Cryo-EM reconstruction showing TTLL6 bound to two PFs in the MT; TTLL6, gold; α-tubulin, purple; β-tubulin, yellow. b, TTLL6 model (X-ray structure PDB 6VZU; ref. 41) fits into the cryo-EM map (Methods). TTLL6 N-, central-, C-, MTBD- and MTBH1-2 domains are blue, magenta, green, orange and cyan, respectively. AlphaFold⁵⁵ was used to model MTBH1 residues 462–478 (not resolved in PDB 6VZU; ref. 41), as the corresponding cryo-EM density was too noisy for de novo building. c, Model of TTLL6 bound to the MT, color-coded as in b. d, Model of TTLL6 as in c, rotated by 180°. Helices in TTLL6 are denoted as α, and helices in tubulin are denoted as H. The interdimer interface (between α′ and β) is indicated by a dashed line.