Skip to main content
. Author manuscript; available in PMC: 2024 Nov 1.
Published in final edited form as: Nat Chem Biol. 2024 Apr 24;20(11):1493–1504. doi: 10.1038/s41589-024-01599-0

Extended Data Fig. 9 ∣. MTBH1-2 promotes tubulin polymerization and mutation of residues in the three cationic clusters impairs this activity.

Extended Data Fig. 9 ∣

a. Quantification of tubulin polymerization over time in the absence or presence of MTBH1-2 and the structure-guided MTBH1-2 mutants. b–f. Representative interference reflection microscopy images from one of two independent experiments of reactions containing 10 μM porcine tubulin without MTBH1-2 (b) or with 2.5 μM MTBH1-2 (c), 2.5 μM MTBH1-2 (K490A/K494A) (d), 25 μM MTBH1-2 (K490A/K494A) (e) and 25 μM MTBH1-2 (K490A/K494A/K498A/K502A) (f). Scale bar, 25 μm.