Fig. 3 ∣. Three MTBH1-2 cationic clusters recognize different MT interfaces.
a, Boxed area 2 in Fig. 2a magnified to show interactions made by MTBH1 with the interdimer interface. b, Boxed area 3 in Fig. 2a magnified to show interactions made by MTBH2 with the β-tubulin subunit at the interdimer interface. c, Top, MTBH1-2 sequence with the three clusters of basic residues highlighted. Bottom, Michaelis–Menten kinetics with taxol-stabilized MTs for TTLL6 mutants in the basic residue clusters. Error bars, s.e.m.; n = 4 independent experiments; apparent KM and kcat values listed on the right. d, Domain schematic of TTLL6 showing the location of cross-linked sites between MTBH1-2 and β-tubulin identified from tandem mass spectrometry analysis (Methods). Red lines indicate cross-linked residues.