Table 2. Macroscopic and microscopic observations in WTEs subjected to necropsy.
WTE no.* | Gender | HPAIV subtype | Observation | Macroscopic finding | Histological analysis | In situ hybridization |
4 | Male | H5N1 | Observed alive exhibiting neurological symptoms and had difficulties flying; later found dead | Hyperaemic skin around eyes and beak; empty gizzard and intestines | Mild, multifocal lymphocytic and histiocytic myocarditis; multifocal splenic necrosis | Positive signal in splenic necroses; few neurons positive in the brain |
6 | Female | H5N5 | Observed alive, exhibiting abnormal behaviour, not able to fly; euthanized | Weight 5.5 kg;sparse content in gizzard | Mild, multifocal lymphocytic and histiocytic myocarditis; multifocal splenic necrosis; mild encephalitis | Few neurons positive in the brain |
8 | Unknown | H5N1 | Found dead | Weight 6.5 kg; dark red lungs; empty gizzard; sparse content in intestines | Multifocal to coalescing myocardial necrosis; mild encephalitis | Positive signal in necrosis, myocytes and vessels in the heart; strong positive signal in neurons and vessels in the brain |
12 | Female | H5N5 | Found dead, with gunshot wounds | Haemorrhages and perforations in muscles related to trauma; empty gizzard | Mild, multifocal lymphocytic and histiocytic myocarditis; multifocal splenic necrosis; mild encephalitis | Analysis not performed |
The WTE no. relates to the numbering in Table S1.