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. 2002 May 15;30(10):e45. doi: 10.1093/nar/30.10.e45

Figure 3.

Figure 3

Overlay of sensorgrams showing the effect of biotin saturated-SA on the binding of aptamer and its specificity. (A) Flow cells 1–3 were pre-coated with 5.7, 4.9 and 4.8 kRU SA, respectively, and flow cell 4 was left as a blank control. Flow cell 2 was saturated with 0.113 kRU of biotin before injecting 200 nM of SA19 aptamer over flow cells 1–4 in series. (B) SA19 aptamer was injected at ∼50 nM over flow cells 1–4 previously coated with BaLgp120 (9 kRU), rat sCD4 (6 kRU), avidin (4.9 kRU) and SA (4.8 kRU), respectively. The arrow indicates the end of injections and start of buffer chase.