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Overlay of sensorgrams showing the effect of mutagenic PCR on binding of SA19 aptamer to SA. Template DNA from various mutagenesis cycles was used to produce 2′-F-RNA. Transcripts (∼65 nM) were injected over sensor chip pre-coated with 4.2 kRU of immobilized SA. The binding to SA was significantly reduced after five cycles of mutagenesis, almost abolished by cycle 10 and completely lost by cycle 15, as compared with the control (0 cycle).
