Figure 2.

DMS activity scores for all measured single amino acid CRX substitutions. Activity scores were normalized to wild-type CRX; the wild-type amino acid at each position is indicated by the gray circle in each column. The average row shows the mean activity score for all substitutions at each position. Disorder, domains, and conservation are shown as in Figure 1. Empty boxes indicate variants not measured in the DMS assay, due to drop-out during the library cloning or variant measurement steps. An interactive version of this figure is available in Supplemental Interactive S1.