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. 2024 Nov 1;15:9448. doi: 10.1038/s41467-024-53894-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 4 — a, b Relative mRNA level of PR1a (a) and PR2 (b) in N. benthamiana plants that were first treated with geldanamycin (GDA) or DMSO and then sprayed with ethanol (control) or SA. c Quantity of TbCSV in N. benthamiana plants that were first sprayed with ethanol (control) or SA and then inoculated with TbCSV+TbCSB+GDA or TbCSV+TbCSB+DMSO. d, e Relative mRNA level of PR1a (d) and PR2 (e) in N. benthamiana plants that were first inoculated with TRV-GFP or TRV-NbHSP90-2 and then sprayed with ethanol (control) or SA. f Quantity of TbCSV in N. benthamiana plants that were first sprayed with ethanol (control) or SA and then inoculated with TbCSV+TbCSB+TRV-GFP or TbCSV+TbCSB+TRV-NbHSP90-2. g, h Relative mRNA level of PR1a (g) and PR2 (h) in N. benthamiana plants that were first inoculated with TRV-GFP or TRV-NbHSP90-10 and then sprayed with ethanol (control) or SA. i Quantity of TbCSV in N. benthamiana plants that were first sprayed with ethanol (control) or SA and then inoculated with TbCSV+TbCSB+TRV-GFP or TbCSV+TbCSB+TRV-NbHSP90-10. j, k Relative mRNA level of PR1a (j) and PR2 (k) in N. benthamiana plants that were first inoculated with TRV-GFP or TRV-NbHSP90-12 and then sprayed with ethanol (control) or SA. l Quantity of TbCSV in N. benthamiana plants that were first inoculated with TbCSV+TbCSB+TRV-GFP or TbCSV+TbCSB+TRV-NbHSP90-12 and then sprayed with ethanol (control) or SA. N = 6–7 samples (2-3 plants per sample) for (a, d, e), 5–7 samples (2–3 plants per sample) for (b, j), 15–16 plants for (c), 14–15 plants for (f), 7-8 samples (2-3 plants per sample) for (g, k), 6–8 samples (2–3 plants per sample) for (h), 12-15 plants for (i), 11–13 plants for l. Data are mean ± SEM. n. s. stands for no significant difference, *P < 0.05, **P < 0.01, and ***P < 0.001 (one-way ANOVA for (a–i); two-sided Student’s t test for j–l). Source data and the exact P values are provided in Source Data file.