Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Nov 1;15:9448. doi: 10.1038/s41467-024-53894-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

PMC Copyright notice

Fig. 6 — a Effect of SA treatment on the degradation of NbNPR3-Flag in semi-in vivo assays. NbNPR3-Flag was expressed in N. benthamiana leaves, proteins extracted, and cycloheximide (CHX) (final concentration 100 µM) and adenosine triphosphate (ATP) (final concentration 20 mM) added. Next, SA or ethanol (control) was added and samples were subjected to incubation. NbNPR3-Flag protein was detected with anti-Flag antibodies. b Effect of SA on the degradation of NbNPR3-Flag in the in vivo assays. NbNPR3-Flag was expressed in N. benthamiana leaves. Leaves were sprayed with SA or ethanol (control) and five hours later collected to determine NbNPR3 levels. c Effect of MG132 on the SA-induced degradation of NbNPR3-Flag in semi-in vivo assays. DMSO was added as control. d Effect of TbCSB βC1-Flag on the SA-induced degradation of NbNPR3-Flag in semi-in vivo assay. GUS-Flag was used as control. NbNPR3-Flag was co-expressed with TbCSB βC1-Flag or GUS-Flag (control). Protein extracts were mixed with CHX, ATP and SA, and then subjected to protein degradation. e Effect of TbCSB βC1-Flag on the SA-induced degradation of NbNPR3-Flag in the in vivo degradation assay. NbNPR3-Flag was co-expressed with TbCSB βC1-Flag and treated with ethanol (control) or SA. Levels of NbNPR3-Flag and TbCSB βC1-Flag were determined five hours later. f Effect of GDA on the suppression of SA-induced degradation of NbNPR3-Flag by TbCSB βC1-Flag in the semi-in vivo degradation assay. DMSO was added as control. g, h Effect of NbHSP90-2-Flag (g) and NbHSP90-10-Flag (h) on the SA-induced degradation of NbNPR3-Flag in the semi-in vivo degradation assay. Ponceau S staining was performed to determine the amount of protein load. Source data are provided in Source Data file.