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. 2024 Nov 1;7:1424. doi: 10.1038/s42003-024-07062-z

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A handC-GFP expressing female flies were immobilised, ventral side up, and either garland (GNCs) or pericardial nephrocytes (PNCs) were isolated using a CellTram equipped with a thin glass capillary. Semi-intact specimen before collagenase treatment with GFP-labelled pericardial cells (B, one nucleus per cell) or garland cells (C, two nuclei per cell). (B’, B”, C’, C”) After collagenase treatment. The dissociated nephrocytes are isolated and collected. Scale bars: 100 µm.