Table 3. Tracking the amount of virus present in cells infected with HRSV following administering shRNA and ribavirin as part of a course of treatment over a period of time .
Vector or ribavirin concentrations | Viral load (copies/mL)/hours post-treatment | |||
24 | 48 | 72 | 96 | |
Untreated control | 4.5 × 105 | 4.2 × 106 | 5.2 × 107 | 6.1 × 108 |
scramble control (20 nM) | 4.4 × 105 | 4.1 × 106 | 5.0 × 107 | 6.0 × 108 |
shRNA targeting HRSV-M gene (10 nM) (5 nM) |
3.7 × 105 | 2.9 × 105 | 7.5 × 104 | 9.2 × 103 |
4.1 × 105 | 3.5 × 105 | 7.9 × 104 | 10.1 × 103 | |
shRNA targeting HRSV-F gene (10 nM) | 3.9 × 105 | 3.2 × 105 | 8.0 × 104 | 10.2 × 103 |
(5 nM) | 4.2 × 105 | 3.7 × 105 | 8.3 × 104 | 10.2 × 103 |
Ribavirin (150 µM) | 3.6 × 105 | 6.2 × 104 | 3.2 × 104 | 9.1 × 103 |
(100 µM) | 4.0 × 105 | 3.0 × 105 | 7.5 × 104 | 9.9 × 103 |
Ribavirin (150 µM) + shRNA targeting HRSV-M gene (10 nM) | 3.4 × 105 | 4.5 × 104 | 2.5 × 104 | 6.9 × 103 |
Ribavirin (150 µM) + shRNA targeting HRSV-F gene (10 nM) | 3.5 × 105 | 5.1 × 104 | 3.0 × 104 | 7.5 × 103 |
shRNA targeting HRSV-M gene (10 nM) + shRNA targeting HRSV-F gene (10 nM) | 3.6 × 105 | 4.7 × 104 | 2.6 × 104 | 7.0 × 103 |