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. 2024 May 14;14(3):696–704. doi: 10.34172/apb.2024.045

Table 3. Tracking the amount of virus present in cells infected with HRSV following administering shRNA and ribavirin as part of a course of treatment over a period of time .

Vector or ribavirin concentrations Viral load (copies/mL)/hours post-treatment
24 48 72 96
Untreated control 4.5 × 105 4.2 × 106 5.2 × 107 6.1 × 108
scramble control (20 nM) 4.4 × 105 4.1 × 106 5.0 × 107 6.0 × 108
shRNA targeting HRSV-M gene (10 nM)
(5 nM)
3.7 × 105 2.9 × 105 7.5 × 104 9.2 × 103
4.1 × 105 3.5 × 105 7.9 × 104 10.1 × 103
shRNA targeting HRSV-F gene (10 nM) 3.9 × 105 3.2 × 105 8.0 × 104 10.2 × 103
(5 nM) 4.2 × 105 3.7 × 105 8.3 × 104 10.2 × 103
Ribavirin (150 µM) 3.6 × 105 6.2 × 104 3.2 × 104 9.1 × 103
(100 µM) 4.0 × 105 3.0 × 105 7.5 × 104 9.9 × 103
Ribavirin (150 µM) + shRNA targeting HRSV-M gene (10 nM) 3.4 × 105 4.5 × 104 2.5 × 104 6.9 × 103
Ribavirin (150 µM) + shRNA targeting HRSV-F gene (10 nM) 3.5 × 105 5.1 × 104 3.0 × 104 7.5 × 103
shRNA targeting HRSV-M gene (10 nM) + shRNA targeting HRSV-F gene (10 nM) 3.6 × 105 4.7 × 104 2.6 × 104 7.0 × 103