Table 5. ANOVA of the best-fitted quadratic model for Vesicle size and entrapment efficiency .
| Source | Vesicle size | Entrapment efficiency | ||||||
| Sum of Squares | df | F value | P value Prob>F | Sum of Squares | df | F value | P value Prob>F | |
| Model | 73440.23 | 9 | 65.27 | < 0.0001* | 1134.96 | 9 | 82.06 | < 0.0001* |
| X1-Bile salt | 17807.91 | 1 | 142.44 | < 0.0001* | 430.48 | 1 | 280.11 | < 0.0001* |
| X2-Edge activator | 1846.90 | 1 | 14.77 | 0.0085* | 9.67 | 1 | 6.29 | 0.0460* |
| X3-Surfactant | 24877.81 | 1 | 199.00 | < 0.0001* | 231.33 | 1 | 150.52 | < 0.0001* |
| X1X2 | 78.58 | 1 | 0.63 | 0.4581** | 9.74 | 1 | 6.34 | 0.0454* |
| X1X3 | 1541.83 | 1 | 12.33 | 0.0126* | 36.62 | 1 | 23.83 | 0.0028* |
| X2X3 | 661.17 | 1 | 5.29 | 0.0611** | 37.10 | 1 | 24.14 | 0.0027* |
| X12 | 19.58 | 1 | 0.16 | 0.7060** | 0.21 | 1 | 0.14 | 0.7258** |
| X22 | 1243.64 | 1 | 9.95 | 0.0197* | 1.99 | 1 | 1.30 | 0.2982** |
| X32 | 2903.35 | 1 | 23.22 | 0.0029* | 23.28 | 1 | 15.15 | 0.0081* |
| Residual | 750.10 | 6 | 9.22 | 6 | - | - | ||
| Lack of Fit | 737.60 | 5 | 11.80 | 0.2173* | 8.72 | 5 | 3.49 | 0.3847** |
| Pure Error | 12.50 | 1 | - | - | 0.50 | 1 | - | - |
| Cor Total | 74190.33 | 15 | - | - | 1144.18 | 15 | - | - |
*P < 0.05 = Significant, **P > 0.05 = Non-significant.