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. 2024 Oct 15;5(4):103393. doi: 10.1016/j.xpro.2024.103393
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Anti-CD31 antibody (dilution: 1:200) Invitrogen Cat# ENMA3100
Anti-connexin43 antibody (dilution: 1:200) Invitrogen Cat# 71-070-0
Anti F-actin antibody (dilution: 1:200) Invitrogen Cat# MA1-80729
DAPI (dilution: 1:1000) Invitrogen Cat# R37606
Polyclonal anti-Staphylococcus aureus antibody (dilution: 1:200) Bio-Rad Cat# 0300-0084
Alexa Fluor 488 polyclonal antibody (dilution: 1:200) Invitrogen Cat# A-11094
Goat anti-rabbit IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 555 (dilution: 1:200) Invitrogen Cat# A-21428

Bacterial and virus strains

Staphylococcus aureus (S. aureus) American Type Cell Culture (ATCC) Cat# 43300

Chemicals, peptides, and recombinant proteins

Gelatin from porcine skin, Bloom 300 Sigma-Aldrich Cat: G2500-1KG
Methacrylic anhydride MilliporeSigma Cas: 760-93-0
Lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) MilliporeSigma Cas: 85073-19-4
Superparamagnetic iron oxide nanoparticles (SPIONs) Micromod Partikeltechnologie GmbH 45-00-252
Antibiotic-Antimycotic Thermo Fisher Scientific 15240062
VascuLife VEGF Endothelial Medium Complete Kit Lifeline Cell Technology Cat# LL-0003
Eagle’s minimum essential medium ATCC Cat# 30-2003
Fetal bovine serum (FBS) ATCC Cat# 30-2021
Penicillin-Streptomycin solution 100x ATCC Cat# 30-2300
Phosphate-buffered saline (PBS), 1X without calcium and magnesium Corning Cat # MT21040CM
Trypsin-EDTA (0.05%), phenol red Gibco Cat# 25300054
Trypan blue Bio-Rad Cat# 1450021
AlamarBlue cell viability reagent Bio-Rad Cat# BUF012B
Paraformaldehyde (PFA) 4% Thermo Fisher Scientific J61899.AP
Luria-Bertani (LB) medium Fisher Scientific MP113002036
Sodium hydroxide (NaOH) Sigma-Aldrich 221465-500G
Agarose Sigma-Aldrich Cat# A9539-100G
10% neutral buffered formalin VWR International, Inc. Cat# 89370-094
Triton X-100 IBI Scientific Cat# IB07100
Blocker bovine serum albumin (BSA) VWR International Cat# PI37535
Antifade mounting medium Fisher Scientific Cat# D1306

Experimental models: Cell lines

Human umbilical vein endothelial cells (HUVECs) Lifeline Cell Technology Cat# FC-0044
NIH/3T3 fibroblasts ATCC Cat# CRL-1658

Other

500 mL Erlenmeyer flask VWR 75804-644
Crystalizing dish VWR 470346-184
Magnetic heater/stirrer Sigma-Aldrich Z671762-1EA
SnakeSkin dialysis tubing (dialysis tubing, 10K MWCO, 35 mm I.D.) Thermo Fisher Scientific 88245
Freeze dryer −84°C Labconco N/A
Bright-field microscope
BioAssemblyBot 400 Advanced Solutions N/A
BioAssemblyBot 10 mL cartridges Advanced Solutions ASLS-0000133
Mach-1 micromechanical system Biomomentum N/A
Spherical indenter (D = 0.5 mm) Biomomentum Cat# MA034
Motion controller, ESP301, 3-Axis, GPIB, USB, RS232 Newport Cat #ESP301-3G
Kimwipes delicate task wipers Kimberly-Clark Cat# 34155
T75 ventilated flask VWR 10062-860
6-well cell culture plate Fisher Scientific 07-200-80
10-cm Petri dish VWR 10416-320
96-well plate VWR 10861-696
Centrifugal tube-15 mL (conical tube) Greiner Cat# 188261
Centrifugal tube-50 mL (conical tube) Greiner Cat# 210261
Biosafety (laminar flow) cabinet N/A N/A
Incubator with humidified atmosphere, at 37°C and 5% CO2 N/A N/A
Water bath with controlled temperature N/A N/A
Centrifuge N/A N/A
Confocal microscope Olympus Fv1000
Microplate reader BioTek Cat# 18531
Vibrating microtome (vibratome) Leica VT1200
Tissue culture plate Sigma-Aldrich CLS430196
Glass slides VWR 48311–703
Glass coverslips VWR 10118–642
Nail polish N/A N/A