Antibodies |
|
Anti-CD31 antibody (dilution: 1:200) |
Invitrogen |
Cat# ENMA3100 |
Anti-connexin43 antibody (dilution: 1:200) |
Invitrogen |
Cat# 71-070-0 |
Anti F-actin antibody (dilution: 1:200) |
Invitrogen |
Cat# MA1-80729 |
DAPI (dilution: 1:1000) |
Invitrogen |
Cat# R37606
|
Polyclonal anti-Staphylococcus aureus antibody (dilution: 1:200) |
Bio-Rad |
Cat# 0300-0084 |
Alexa Fluor 488 polyclonal antibody (dilution: 1:200) |
Invitrogen |
Cat# A-11094 |
Goat anti-rabbit IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 555 (dilution: 1:200) |
Invitrogen |
Cat# A-21428 |
|
Bacterial and virus strains |
|
Staphylococcus aureus (S. aureus) |
American Type Cell Culture (ATCC) |
Cat# 43300 |
|
Chemicals, peptides, and recombinant proteins |
|
Gelatin from porcine skin, Bloom 300 |
Sigma-Aldrich |
Cat: G2500-1KG |
Methacrylic anhydride |
MilliporeSigma |
Cas: 760-93-0 |
Lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) |
MilliporeSigma |
Cas: 85073-19-4 |
Superparamagnetic iron oxide nanoparticles (SPIONs) |
Micromod Partikeltechnologie GmbH |
45-00-252 |
Antibiotic-Antimycotic |
Thermo Fisher Scientific |
15240062 |
VascuLife VEGF Endothelial Medium Complete Kit |
Lifeline Cell Technology |
Cat# LL-0003 |
Eagle’s minimum essential medium |
ATCC |
Cat# 30-2003 |
Fetal bovine serum (FBS) |
ATCC |
Cat# 30-2021 |
Penicillin-Streptomycin solution 100x |
ATCC |
Cat# 30-2300 |
Phosphate-buffered saline (PBS), 1X without calcium and magnesium |
Corning |
Cat # MT21040CM |
Trypsin-EDTA (0.05%), phenol red |
Gibco |
Cat# 25300054 |
Trypan blue |
Bio-Rad |
Cat# 1450021 |
AlamarBlue cell viability reagent |
Bio-Rad |
Cat# BUF012B |
Paraformaldehyde (PFA) 4% |
Thermo Fisher Scientific |
J61899.AP |
Luria-Bertani (LB) medium |
Fisher Scientific |
MP113002036 |
Sodium hydroxide (NaOH) |
Sigma-Aldrich |
221465-500G |
Agarose |
Sigma-Aldrich |
Cat# A9539-100G |
10% neutral buffered formalin |
VWR International, Inc. |
Cat# 89370-094 |
Triton X-100 |
IBI Scientific |
Cat# IB07100 |
Blocker bovine serum albumin (BSA) |
VWR International |
Cat# PI37535 |
Antifade mounting medium |
Fisher Scientific |
Cat# D1306 |
|
Experimental models: Cell lines |
|
Human umbilical vein endothelial cells (HUVECs) |
Lifeline Cell Technology |
Cat# FC-0044 |
NIH/3T3 fibroblasts |
ATCC |
Cat# CRL-1658 |
|
Other |
|
500 mL Erlenmeyer flask |
VWR |
75804-644 |
Crystalizing dish |
VWR |
470346-184 |
Magnetic heater/stirrer |
Sigma-Aldrich |
Z671762-1EA |
SnakeSkin dialysis tubing (dialysis tubing, 10K MWCO, 35 mm I.D.) |
Thermo Fisher Scientific |
88245 |
Freeze dryer −84°C |
Labconco |
N/A |
Bright-field microscope |
|
|
BioAssemblyBot 400 |
Advanced Solutions |
N/A |
BioAssemblyBot 10 mL cartridges |
Advanced Solutions |
ASLS-0000133 |
Mach-1 micromechanical system |
Biomomentum |
N/A |
Spherical indenter (D = 0.5 mm) |
Biomomentum |
Cat# MA034 |
Motion controller, ESP301, 3-Axis, GPIB, USB, RS232 |
Newport |
Cat #ESP301-3G |
Kimwipes delicate task wipers |
Kimberly-Clark |
Cat# 34155 |
T75 ventilated flask |
VWR |
10062-860 |
6-well cell culture plate |
Fisher Scientific |
07-200-80 |
10-cm Petri dish |
VWR |
10416-320 |
96-well plate |
VWR |
10861-696 |
Centrifugal tube-15 mL (conical tube) |
Greiner |
Cat# 188261 |
Centrifugal tube-50 mL (conical tube) |
Greiner |
Cat# 210261 |
Biosafety (laminar flow) cabinet |
N/A |
N/A |
Incubator with humidified atmosphere, at 37°C and 5% CO2
|
N/A |
N/A |
Water bath with controlled temperature |
N/A |
N/A |
Centrifuge |
N/A |
N/A |
Confocal microscope |
Olympus |
Fv1000 |
Microplate reader |
BioTek |
Cat# 18531 |
Vibrating microtome (vibratome) |
Leica |
VT1200 |
Tissue culture plate |
Sigma-Aldrich |
CLS430196 |
Glass slides |
VWR |
48311–703 |
Glass coverslips |
VWR |
10118–642 |
Nail polish |
N/A |
N/A |