Table 3.
Criteria for embryo evaluation and cryopreservation at the blastocyst stage
| Total (n=345) | With time-lapse technology (n=195) | Without time-lapse technology (n=150) | p valuea | |
|---|---|---|---|---|
| Embryo evaluation | ||||
| Gardner's classification, n (%) | 334 (96.8) | 187 (95.9) | 147 (98.0) | .2705 |
| In-house grading systems, n (%) | 43 (12.5) | 25 (12.8) | 18 (12.0) | .8191 |
| iDAScore, n (%) | 6 (1.7) | 6 (3.1) | – | |
| KIDScore, n (%) | 4 (1.2) | 4 (2.1) | – | |
| Observation point | ||||
| Size of blastocysts, n (%) | 239 (69.3) | 139 (71.3) | 100 (66.7) | .3570 |
| Size of blastocoel, n (%) | 170 (49.3) | 91 (46.7) | 79 (52.7) | .2691 |
| Presence of hatching, n (%) | 115 (33.3) | 58 (29.7) | 57 (38.0) | .1068 |
| Size of inner cell mass, n (%) | 253 (73.3) | 147 (75.4) | 106 (70.7) | .3259 |
| No. of cells in inner cell mass, n (%) | 252 (73.0) | 142 (72.8) | 110 (73.3) | .9153 |
| Density of inner cell mass, n (%) | 243 (70.4) | 139 (71.3) | 104 (69.3) | .6942 |
| Uniformity of cells in inner cell mass, n (%) | 137 (39.7) | 75 (38.5) | 62 (41.3) | .5889 |
| No. of cells in trophectoderm, n (%) | 316 (91.6) | 186 (95.4) | 130 (86.7) | .0038 |
| Density of cells in trophectoderm, n (%) | 254 (73.6) | 147 (75.4) | 107 (71.3) | .3973 |
| Uniformity of cells in trophectoderm, n (%) | 200 (58.0) | 111 (56.9) | 89 (59.3) | .6530 |
| Presence of fragments, n (%) | 133 (38.6) | 68 (34.9) | 65 (43.3) | .1094 |
| Degree of fragments, n (%) | 128 (37.1) | 71 (36.4) | 57 (38.0) | .7619 |
| Morphokinetics, n (%) | 230 (66.7) | 155 (79.5) | 75 (50.0) | <.0001 |
| Morphology of the oocyte, n (%) | 1 (0.3) | – | 1 (0.7) | |
| Criteria for cryopreservation | ||||
| Gardner's classification, n (%) | 323 (93.6) | 179 (91.8) | 144 (96.0) | .1130 |
| In-house grading systems, n (%) | 64 (18.6) | 42 (21.5) | 22 (14.7) | .1036 |
| iDAScore n (%) | 3 (0.9) | 3 (1.5) | – | |
| KIDScore, n (%) | 2 (0.6) | 2 (1.0) | – | |
| Physician's decision, n (%) | 2 (0.6) | 1 (0.5) | 1 (0.7) | .8520 |
| No criteria, n (%) | 4 (1.2) | 1 (0.5) | 3 (2.0) | .2008 |
a
Comparison between facilities with time-lapse technology and those without time-lapse technology.
Yamada. Use of time-lapse technology on fertilization verification, embryo evaluation, and utilization. Am J Obstet Gynecol MFM 2024.