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. 2024 Nov 2;17(11):e70039. doi: 10.1111/1751-7915.70039

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© 2024 The Author(s). Microbial Biotechnology published by John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Psilocybin production in S. cerevisiae mutant strains. (A) The phenotypic analysis of l‐tryptophan catabolism‐repressed S. cerevisiae confirmed their sensitivity to high l‐tryptophan (Trp) concentrations. (B) Genetic map of the expression plasmid pYes‐psi carrying the psi cluster expressed as a polycistron under the control of the constitutive TEF1 promoter. (C) Production of psilocybin, psilocin and baeocystin in S. cerevisiae wild type and tryptophan catabolism‐repressed mutants after 48 h of cultivation.