FIGURE 6.

Process characterization and respiratory activity monitoring. (A) Online data of oxygen transfer rate (OTR) and carbon dioxide transfer rate (CTR) is shown for the A. nidulans double mutant ΔaroH1/ΔaroH2 and the control strain tJF03, both expressing the psi cluster as a polycistron. Induction was performed at 31 h upon decrease of metabolic activity observed by the decline in OTR signal. For improved enzyme production, the temperature was shifted to 30°C as indicated. (B) Offline data of glucose concentration, cell dry weight (CDW) and pH progression is shown over the cultivation time for both strains. (C) Production of psilocybin, psilocin and baeocystin over the time of cultivation for the wild type (tJF03) and double mutant backgrounds. Data are presented as averages from duplicate shake flask cultivations and triplicate product quantification with standard errors presented as error bars.