Fig. 8.

FTH1 and FTL were the downstream factors of FGF21 that exerted the inhibitory effect on ferroptosis in DCM. A-E, Transfection of FGF21-plasmid and FTH1-SI or/and FTL-SI for 48 h and HG/HF incubation for the following 48 h, levels of cellular activity (A), representative immunoblot images and statistical results of GPX4 (B), MDA (C), Ptgs2 mRNA (D), and representative LSCM images of mitochondria membrane potential, lipid ROS and intracellular Fe²⁺ were measured using JC1 dye, BODIPY™ 581/591 C11 and FerroOrange respectively (E) in primary cardiomyocytes (per image represents more than 15 cells). Scale bar: 50 μm. F-J, Transfection of FGF21-plasmid and FTH1-siRNA or/and FTL-siRNA for 48 h and HG/HF incubation for the following 48 h, levels of cellular activity (F), representative immunoblot images and statistical results of GPX4 (G), MDA (H) and Ptgs2 mRNA (I), and representative LSCM images of mitochondria membrane potential, lipid ROS and intracellular Fe²⁺ were measured using JC1 dye, BODIPY™ 581/591 C11 and FerroOrange respectively (J) in primary cardiomyocytes (per image represents more than 15 cells). Scale bar: 50 μm. *P < 0.05