Fig. 5.

Tissue-specific consequences of Trps1 deficiency on expression of proteins important for cementum formation and alveolar bone. Representative microscopic images of IHC on the tooth root with adjacent periodontal ligament and alveolar bone in 4wk old WT and Trps1^Col1a1 cKO mice. (A) IHC for Osx showing fewer Osx-positive cells around the acellular and cellular cementum, and on the alveolar bone surface in Trps1^Col1a1 cKO mice compared to WT mice. (B) IHC for TNAP showing lower TNAP levels around both acellular and cellular cementum and on the alveolar bone surface in Trps1^Col1a1 cKO mice compared to the WT. (C) IHC for Bsp showing comparable levels of Bsp in acellular and cellular cementum of WT and Trps1^Col1a1 cKO mice, and notably lower levels of Bsp in the alveolar bone of Trps1^Col1a1cKO mice in comparison with WT. Arrows point at the irregularity of the acellular cementum in the Trps1^Col1a1 cKO mice. AC–acellular cementum; CC–cellular cementum; D–dentin; AB–alveolar bone