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. 2024 Nov 2;14:26390. doi: 10.1038/s41598-024-77733-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 13 — Graphical summary. After establishing the xenograft mouse model, human leukemia cells from spleen, BM, and CNS were isolated and, together with cells from nine individual BCP ALL cell lines (NALM-6, REH, RS4;11, KOPN-8, UoCB6, RCH-ACV, MHH-CALL2, EU3, and HAL-01) and PBMCs derived from six human healthy donors, were subjected to miRNA isolation and cDNA synthesis. The expression levels of six standards (5sRNA, SNORD44, RNU6, RNU1A1, miR-103a-3p, and miR-532-5p) in all samples were analyzed by qPCR. Four different algorithms (geNorm, BestKeeper, Normfinder, ΔCT) were applied, identifying miR-103a-3p and miR-532-5p as the most reliable references to be used in accurate qPCR normalization in miRNAs studies in BCP ALL xenografts. NOD/SCID/huALL: non-obese diabetic/severe combined immunodeficiency/human acute lymphoblastic leukemia; BM: bone marrow; CNS: central nervous system; PBMC: peripheral blood mononuclear cell. Created in BioRender. Meyer, L. (2024) https://BioRender.com/z55z209