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. 2024 Nov 2;14:26390. doi: 10.1038/s41598-024-77733-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Stability of miRNA references in PDX ALL samples and ALL cell lines of the identification cohort. Expression stability within the cohort samples was analyzed applying the Normfinder (A and B; A: samples clustered in 1 group; B: samples sorted in 4 groups according to spleen-, BM-, CNS-derived PDX samples, and ALL cell lines), BestKeeper (C), geNorm (D), or ∆CT algorithm (E and F). For A-E: decreasing values indicate increasing stability. F: ∆CT calculations based on CT[reference 1] – CT[reference 2]; mean CT values with min to max whiskers are shown. PDX: patient-derived xenograft; ALL: acute lymphoblastic leukemia.