Figure 3.

Grafts of human cardiac troponin I (cTnI)+ hiPSC-CLs across the right ventricular myocardium. (A) Representative images of the right ventricular myocardium of all hiPSC-CL injected animals. Cardiac tissue was immunostained for human cTnI (brown) and counterstained with hematoxylin (blue). (B) High-magnification image of area demarcated by black rectangle in HD12-1. Scale bar: (A) 1,000 µm, (B) 100 µm. (C) Heat map of human cTnI+ graft area (mm2) per subject across cardiac tissue levels. Each heart was sliced in 4-mm blocks from the apex to the base, and tissue from each block was immunolabeled for human cTnI to detect grafted cells across the entire right ventricle. The number of blocks depended on individual animal size; gray boxes indicate no right ventricle tissue at this level. White, empty boxes indicate no human cTnI+ graft detected. *, some human cTnI was detected, but graft size was not quantified due to lack of perfusion impacting immunolabeling. DNC, did not complete study.