Figure 3.
Genetic interaction of SAS-negative regulators in the shade-induced hypocotyl elongation. Difference of hypocotyl length (HYP) in simulated shade, W + FR (HYPW + FR) and W (HYPW) of Col-0, A) phyA-211, hfr1-5, phyA-211 hfr1-101, B) hy5-2, hfr1-5, hy5-2 hfr1-1, C) phyA-501, hy5-2, and phyA-501 hy5-2. Seedlings were grown as in Fig. 2A. Means and Se of 3 independent replicates were used to calculate the shown values of HYPW + FR − HYPW and to propagate the Se. Error bars are the propagated Se. Black asterisks indicate significant differences (based on the 2-way ANOVA) between the mutant and wild-type genotypes in response to simulated shade (**P < 0.01). In A) and C), red asterisks indicate significant differences (based on the 2-way ANOVA) between the double mutants and phyA single genotypes in response to simulated shade (**P < 0.01; ns, not significant).