FIGURE 6.

In vivo experiments to confirm the effect of ACTN4 on the dissemination of Ty‐82 cells. (A) Schematic illustration of the injection of Ty‐82 cells into the thoracic cavity of a mouse to induce dissemination. Black triangles indicate the disseminated lesion on the lung surface. (B, C) Representative hematoxylin–eosin (H&E) findings of lungs from sacrificed mice. Disseminated lesions were observed on the surface of the lungs of mice injected with control Ty‐82 cells (red arrows). Scale bars indicate 100 μm. (D) Comparison of the number of disseminated lesions per mouse between control Ty‐82 and ACTN4 KD. *p < 0.01.