Table 1.

Characterization and validation.

Classification	Test	Result	Data
Morphology	Photography	Visual record of the line: normal	Fig. 1 panel A
Phenotype	Quantitative analysis: RT-qPCR	Positive expression of pluripotency markers NANOG and SOX2 in the two FH iPSC lines and absent in differentiated CMs	Fig. 1 panel B
	Qualitative analysis: Immunofluorescence staining	Positive expression of pluripotency markers: OCT3/4, NANOG, and SOX2 in the two FH iPSC lines	Fig. 1 panel C
Genotype	Whole genome array (KaryoStat™ Assay) Resolution 1–2 Mb	Normal karyotype: 46, XY for line SCVIi108-A and 46, XX for line SCVIi109-A	Fig. 1 panel F
Identity	Microsatellite PCR (mPCR) or	N/A	N/A
	STR analysis	16 loci tested match well	submitted in archive with journal
Mutation analysis (IF APPLICABLE)	Sequencing Southern blot or WGS	Heterozygous mutation N/A	Fig. 1 panel E N/A
Microbiology and virology	Mycoplasma	Mycoplasma testing by luminescence: Negative	Fig. 1 panel H
Differentiation potential	Directed differentiation	Positive IF staining of three germ layer markers	Fig. 1 panel D
List of recommended germ layer markers	Expression of these markers has to be demonstrated at mRNA (RT qPCR) or protein (IF) levels, at least 2 markers need to be shown per germ layer	Ectoderm: PAX6, OTX2 Endoderm: SOX17, FOXA2 Mesoderm: Brachyury, TBX6	Fig. 1 panel D
Donor screening (OPTIONAL)	HIV 1 + 2 Hepatitis B, Hepatitis C	N/A	N/A
Genotype additional info (OPTIONAL)	Blood group genotyping	N/A	N/A
	HLA tissue typing	N/A	N/A