Figure 6.

Paquinimod treatment alters the migration capacity of Ly6C^high monocytes sorted from bone marrow. (A, B) Quantification of migrated Ly6C^high bone marrow monocytes into the lower chamber of the Boyden chamber assay. Cells were treated with or without various doses of the inhibitor Paquinimod (Paq). Migration was assessed after 24 hours of incubation at 37°C and 5% CO2. (A) Migration response of Ly6C^high bone marrow monocytes in response to medium alone (–) or S100A9 (9 µg/ml, +). (B) Migration response of Ly6C^high bone marrow monocytes in response to medium alone (–) or CT26 tumor cell line supernatant (CT26 sup, +). Figure shows the number of migrated cells stained with NucBlue. Results are expressed as mean ± SD of pooled data from three independent experiments. Statistically significant differences between treatment groups are denoted * or ** depending on p-values as described in methods section.