Figure 4 .

FOXM1 induces MTX resistance by enhancing autophagy in OS cells

(A,C) LC3-II protein expression in FOXM1-overexpressing cells (A) or FOXM1-knockdown cells (C) was detected by western blot analysis after treatment with 50 μM MTX for 24 h. (B,D) The expression of LC3B was also detected by immunofluorescence staining in FOXM1-overexpressing cells (B) or FOXM1-knockdown cells (D). Scale bar: 100 μm. After treatment with 50 μM MTX for 24 h in the presence or absence of CQ (20 μM), apoptosis was detected by flow cytometry in FOXM1-overexpressing cells (E). n=3, * P<0.05, ** P<0.01.