Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1984 Jan 15;217(2):419–425. doi: 10.1042/bj2170419

cis-Unsaturated fatty acids uncouple mitochondria and stimulate glycolysis in intact lymphocytes.

P Arslan, A N Corps, T R Hesketh, J C Metcalfe, T Pozzan
PMCID: PMC1153232  PMID: 6696740

Abstract

In intact lymphocytes, linoleic acid acts as a typical mitochondrial uncoupler: it substantially decreases the mitochondrial membrane potential and the cellular ATP level, while stimulating oxygen consumption and glycolysis. Under these conditions the inhibition of cellular functions by linoleic acid cannot be attributed to its postulated effects on lipid domains in the plasma membrane.

Full text

PDF
420

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arslan P., Montecucco C., Celi D., Pozzan T. Effect of monovalent cation ionophores on lymphocyte cellular metabolism. Biochim Biophys Acta. 1981 Apr 22;643(1):177–181. doi: 10.1016/0005-2736(81)90230-3. [DOI] [PubMed] [Google Scholar]
  2. Corps A. N., Pozzan T., Hesketh T. R., Metacalfe J. C. cis-Unsaturated fatty acids inhibit cap formation on lymphocytes by depleting cellular ATP. J Biol Chem. 1980 Nov 25;255(22):10566–10568. [PubMed] [Google Scholar]
  3. Felber S. M., Brand M. D. Factors determining the plasma-membrane potential of lymphocytes. Biochem J. 1982 May 15;204(2):577–585. doi: 10.1042/bj2040577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Goldstein S., Korczack L. B. Status of mitochondria in living human fibroblasts during growth and senescence in vitro: use of the laser dye rhodamine 123. J Cell Biol. 1981 Nov;91(2 Pt 1):392–398. doi: 10.1083/jcb.91.2.392. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hoek J. B., Nicholls D. G., Williamson J. R. Determination of the mitochondrial protonmotive force in isolated hepatocytes. J Biol Chem. 1980 Feb 25;255(4):1458–1464. [PubMed] [Google Scholar]
  6. Hoover R. L., Klausner R., Karnovsky M. J. Inhibition of cap formation on lymphocytes by free fatty acids is not mediated by a depletion of ATP. J Biol Chem. 1982 Mar 10;257(5):2151–2154. [PubMed] [Google Scholar]
  7. Hume D. A., Radik J. L., Ferber E., Weidemann M. J. Aerobic glycolysis and lymphocyte transformation. Biochem J. 1978 Sep 15;174(3):703–709. doi: 10.1042/bj1740703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Johnson L. V., Walsh M. L., Chen L. B. Localization of mitochondria in living cells with rhodamine 123. Proc Natl Acad Sci U S A. 1980 Feb;77(2):990–994. doi: 10.1073/pnas.77.2.990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Karnovsky M. J., Kleinfeld A. M., Hoover R. L., Klausner R. D. The concept of lipid domains in membranes. J Cell Biol. 1982 Jul;94(1):1–6. doi: 10.1083/jcb.94.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Klausner R. D., Bhalla D. K., Dragsten P., Hoover R. L., Karnovsky M. J. Model for capping derived from inhibition of surface receptor capping by free fatty acids. Proc Natl Acad Sci U S A. 1980 Jan;77(1):437–441. doi: 10.1073/pnas.77.1.437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pozzan T., Arslan P., Tsien R. Y., Rink T. J. Anti-immunoglobulin, cytoplasmic free calcium, and capping in B lymphocytes. J Cell Biol. 1982 Aug;94(2):335–340. doi: 10.1083/jcb.94.2.335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Pozzan T., Corps A. N., Montecucco C., Hesketh T. R., Metcalfe J. C. Cap formation by various ligands on lymphocytes shows the same dependence on high cellular ATP levels. Biochim Biophys Acta. 1980 Nov 18;602(3):558–566. doi: 10.1016/0005-2736(80)90334-x. [DOI] [PubMed] [Google Scholar]
  13. Rink T. J., Montecucco C., Hesketh T. R., Tsien R. Y. Lymphocyte membrane potential assessed with fluorescent probes. Biochim Biophys Acta. 1980;595(1):15–30. doi: 10.1016/0005-2736(80)90243-6. [DOI] [PubMed] [Google Scholar]
  14. Roos D., Loos J. A. Changes in the carbohydrate metabolism of mitogenically stimulated human peripheral lymphocytes. II. Relative importance of glycolysis and oxidative phosphorylation on phytohaemagglutinin stimulation. Exp Cell Res. 1973 Mar 15;77(1):127–135. doi: 10.1016/0014-4827(73)90561-2. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES