Fig. 7.

Gpx1 palmitoylation facilitates the downregulation of ROS.

(A–B) Cultured WT and Gpx1-KO HUVECs were treated with H₂O₂ for varying time periods, stained with CellROX® Deep Red for Fluorescence Imaging (A), and quantified (B). (C–D) Gpx1-KO HUVECs rescued with Gpx1 or Gpx1-2CS were treated with H₂O₂ for varying time periods, stained with CellROX® Deep Red for Fluorescence Imaging (C), and quantified (D). (E–F) Cultured WT HUVECs were treated with H₂O₂ for varying time periods, and collected for the evaluation of protein palmitoylation by ABE assay (E), with quantification (F). (G) The Gpx1 activity was measured in Gpx1-KO HUVECs rescued with either WT Gpx1 or Gpx1-2CS. Blots and images shown are representative, and data represents the mean ± S.E.M. from three biological replicates or otherwise indicated. Statistical significance is denoted as ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001, determined by student t-test.