Table 1.

List of primers used and thermocycler setting¹ used for the amplification of the virulence genes.

Set	Target genes	Accession No.	Primer name	Sequence (5′–3′)	Length (nt)	Tm (°C)	Amplicon Size(bp)	References
A2	ciaB	NP_282066	ciaB.F335	GGTCTAACTTCATCAACCCTTTGC	24	62.9	658	Poudel et al. (2022)
			ciaB.R992	CTCATGCGGTGGCATTAGAATG	22	62.7
	cadF	NP_282616	cadF.F20	GCATCCACTCTTCTATTATCCGC	23	62.8	543	Poudel et al. (2022)
			cadF.R562	ATTCCGTCTTAGTGATTCTTTGGC	24	61.2
	cdtA	NP_281292	cdtA.F3	ATCGTACCTCTCCTTGGCG	19	62.3	440	Poudel et al. (2022)
			cdtAR442	CGGAGCAGCTTTAACGGTTTG	21	62.6
	cdtC	NP_281290	cdtC.F260	GCTCCAAAGGTTCCATCTTCTAAG	24	62.9	263	Poudel et al. (2022)
			cdtC.R522	GCAACTCCTACTGGAGATTTGAAAG	25	62.9
	cdtB	NP_281291	cdtB.F152	GCTTGAGTTGCGCTAGTTGG	20	62.4	180	Poudel et al. (2022)
			cdtB.R331	TGGAGGAACAGATGTAGGAGC	21	62.6
B2	virB9	YP_980061	virB9.F429	AAGAACACGCTTTGCAATGGC	21	60.6	535	Poudel et al. (2022)
			virB9.R964	CGATGATCCTAGTCTCTACTGGAC	24	64.6
	pebA	NP_282073	pebA.F40	GCTCTAGGTGCTTGTGTTGC	20	62.4	436	Poudel et al. (2022)
			pebA.R476	GTAGTTGCAGCTTGAGCCAC	20	62.4
	porA	NP_282406	porA.F740	TCAACTGGACACTTGAAGGTGC	22	62.7	342	Poudel et al. (2022)
			porA.R1082	CCACCATATACGAAGTCAGCACC	23	64.8
	flhB	NP_281526	flhB.F531	GGTTGCACAGCTTACTTGGC	20	62.4	257	Poudel et al. (2022)
			flhB.R788	ACATCCGCACCTGCAACATC	20	62.4
	jlpA	NP_282133	jlpA.F998	GCACACAGGGAATCGACAGC	20	64.5	119	Poudel et al. (2022)
			jlpA.R1116	AAATGACGCTCCGCCCATTAAC	22	62.7
C3	flaAB	NP_282485 (flaA) NP_282484 (flaB)	flaA.R1094	CAGTTGGAACAGGACTTGGAG	21	62.6	∼1500	Poudel et al. (2022)
			flaB.R253	GCTCATCCATAGCCTTATCAGCAG	24	64.6
	pldA	Part of NC_002163	pldA.F422	GCCTATACTCAAACTTCTTGGTGG	24	60.6	499	Poudel et al. (2022)
			pldA.R940	AGTCTATAAGGCTTTCTCCATAGCC	25	62.9
	flgB	NP_281712	flgB.F25	GAACTGGTCACTGGTGCTTTAGC	23	64.6	224	Poudel et al. (2022)

¹PCR thermocycler condition was initial denaturation at 95 °C for 3 min followed by 35 cycles of 95° C for 30 s, 60° C for 30 s, 72° C for 60 s, and a final extension step of 72° C for 5 min.

²Virulence genes run as a pentaplex PCR.

³Virulence gene run individually for PCR.