Table 2.

List of primers used and thermocycler setting¹ used for the amplification of the antimicrobial resistance genes.

Set	Target genes	Accession No.	Orientation	Sequence (5′–3′)	Length (nt)	Tm (°C)	Amplicon Size (bp)	Reference
A2	aph (3′)-IIIa	NG_047420	Forward	TGCACTTTGAACGGCATGATG	21	56.5	432	Poudel et al. (2022)
			Reverse	TGTCATACCACTTGTCCGCC	20	57.3
	aph (2″)-Ig	NG_047407	Forward	GATTTACCTGCCTTGATTCCGG	22	56.0	523	Poudel et al. (2022)
			Reverse	TTCGCCGAAATCTTTCCCA	19	54.6
	blaOXA-184	NG_049485	Forward	GCTCTCAAGTGCCTGCTTTT	20	56.0	317	Poudel et al. (2022)
			Reverse	AAATCCAACAATCCAAGCCAAA	22	53.6
	blaOXA-61	NG_049801	Forward	CTTTCTCTCCCGCTTCCACT	20	56.8	203	Poudel et al. (2022)
			Reverse	ACCAATTCTTCTTGCCACTTCTTT	24	55.3
	tet(O)	NG_048260	Forward	AATATTCAGAGAAAAGGCGGCG	22	55.7	686	Poudel et al. (2022)
			Reverse	GCAGCCATAAAGAACCCCCT	20	57.6
B3	ermB	KC575115	Forward	GGGCATTTAACGACGAAACTGG	22	62.7	421	Cheng et al. (2020)

¹PCR thermocycler condition was initial denaturation at 95 °C for 3 min followed by 35 cycles of 95° C for 30 s, 55° C for 30 s, 72° C for 60 s, and a final extension step of 72° C for 5 min.

²Antibiotics resistance genes run as a pentaplex PCR.

³Antibiotics resistance gene run individually for PCR.