Figure 2.

FOXP4 depletion exerts antitumor effects in gastric cancer. A and B, siFOXP4 inhibited cancer cell proliferation and colony formation (n = 3). C, siFOXP4 suppressed gastric cancer cell invasiveness (n = 3). D and E, Western blot analysis of cell cycle–associated and apoptosis-associated proteins after FOXP4 knockdown. F, FOXP4 knockdown–induced apoptosis was confirmed by flow cytometry (n = 3). G, Representative patient-derived organoid images with shFOXP4-mediated knockdown. Scale bar, 50 μm. H, Subcutaneous injection of FOXP4-depleted gastric cancer cells formed smaller xenografts than the control group mice. I, According to IC₅₀ displayed, siFOXP4 increased the 5-FU sensitivity. J, FOXP4 deletion and 5-FU combination suppressed peritoneal metastasis and prolonged survival time of mice. *, P < 0.05; **, P < 0.01; ***, P < 0.001.