Figure 4.

FOXP4 overexpression promotes tumor growth and partially rescues the suppressive effects of YAP1 knockdown. A and B, FOXP4 overexpression enhanced the proliferation and colony formation ability of MKN7 cells. C, FOXP4-overexpressed MKN7 cells showed enhanced migration and invasion abilities. D and E, FOXP4 overexpression significantly increased the tumor formation ability of MKN7 cells. F, Western blot analysis revealed that the expression level of stemness markers was upregulated in the FOXP4 overexpression cells. G, Stronger peritoneal metastasis signals were detected in mice injected with FOXP4-overexpressed MKN7 cells compared with the empty vector group. H, Rescue experiments were performed by re-introducing FOXP4 into gastric cancer cells with YAP1 depletion. Re-overexpressing FOXP4 partially abolished the suppressive effects of YAP1 knockdown. I and J, The suppressed proliferation and colony formation ability from YAP1 knockdown were rescued by FOXP4 overexpression. K, The decreased spheroid-forming ability induced by siYAP1 was rescued by FOXP4 overexpression. L, FOXP4 overexpression partially rescued the suppressed cell migration and invasion induced by YAP1 deletion. *, P < 0.05; **, P < 0.01; ***, P < 0.001.