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. 2024 Nov 4;23:297. doi: 10.1186/s12934-024-02557-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 4 — Transcription level changes of duplicated genes and pMMO of CS40. a Transcription level change of pMMO in CS40. b, c, d, e, f The changes of transcription level of genes in duplication region. b AYM39_08385; Malyl-CoA lyase, c AYM39_08395; Malate thiokinase subunit beta, d AYM39_08400; Malate thiokinase subunit alpha, e AYM39_08540; Methenyltetrahydromethanopterin cyclohydrolase, f AYM39_08690; Peroxiredoxin. Transcription level of each gene in CS was set at one for comparison. Transcription level of each gene was normalized using transcription level of 16S rRNA gene. Each data set obtained from three separate experiments