Table 1.
Bacterial strains and plasmids used in this study
| Name | Description | References |
|---|---|---|
| Strains | ||
| E. Coli TOP10 | Cloning host strain; | Invitrogen |
| Methylomonas sp. DH-1 | Wild-type strain, A novel Type I methanotroph isolated from brewery waste sludge | Hur et al. [19] |
| DH-Cre | Methylomonas sp. DH-1 Cre-lox | Kang et al. [29] |
| CS | DH-Cre ΔsdhB-ms-il | This study |
| CS40 | Evolved strain from CS | This study |
| CS401 | CS40 PAYM39_18825-pc | This study |
| CS402 | CS40 PAYM39_18825-ppc | This study |
| CS403 | CS401 PAYM39_18825-ppc | This study |
| CS404 | CS40 Δssadh | This study |
| CS405 | CS403 Δssadh PmxaF-gltA | This study |
| CS40-GFP1 | CS40 Ppc-GFP | This study |
| CS40-GFP2 | CS40 Pppc-GFP | This study |
| CS40-GFP3 | CS40 P18825-GFP | This study |
| Plasmid | ||
| pDCKIU | pUC; Cre-lox system integration | Kang et al. [29] |
| pCM184-F12-sdh-ms-il | pCM184; sdhB deletion, intergration of ms-il from E. coli MG1655 | Nguyen et al. [20] |
| pTOP Blunt V2 | Integration/Deletion vector; AmpR, KanR | Invitrogen |
| pUC19 | Integration/Deletion vector; AmpR | This study |
| pTOP-ssadh | pTOP Blunt V2; ssadh deletion vector | This study |
| pTOP-ssadh-gltA | pTOP Blunt V2; ssadh deletion and gltA overexpression vector | This study |
| pUC19-pc | pUC19; pc promoter exchange vector | This study |
| pUC19-ppc | pUC19; ppc promoter exchange vector | This study |
| pTOP-GFP(Ppc) | pTOP-ssadh; GFP integration with the pc promoter | This study |
| pTOP-GFP(Pppc) | pTOP-ssadh; GFP integration with the ppc promoter | This study |
| pTOP-GFP(P18825) | pTOP-ssadh; GFP integration with the AYM39_18825 promoter | This study |
The following gene abbreviations were used: sdhB, succinate dehydrogenase, gltA, and citrate synthase. The following protein abbreviations were used: ms, malate synthase; il, isocitrate lyase; pc, pyruvate carboxylase; ppc, phosphoenolpyruvate carboxylase; ssadh, succinate semialdehyde dehydrogenase; AmpR, ampicillin resistance protein; KanR, kanamycin resistance protein