Table 5.
Top 20 RNA sequencing data of DH-1 genes for selection of promoter
No | Locus tag | Type | Functions | TPM* (3.5% O2) | TPM* (15% O2) | Fold** |
---|---|---|---|---|---|---|
1 | AYM39_17695 | tmRNA | Transfer-messenger RNA | 190,466 | 185,239 | 0.97 |
2 | AYM39_19775 | Protein | Methane monooxygenase subunit C | 96,356 | 27,806 | 0.29 |
3 | AYM39_20600 | Protein | RNase P RNA component class A | 60,979 | 50,865 | 0.83 |
4 | AYM39_14685 | Protein | Hypothetical protein | 27,449 | 3397 | 0.12 |
5 | AYM39_19765 | Protein | Methane monooxygenase subunit B | 27,086 | 8147 | 0.30 |
6 | AYM39_19770 | Protein | Methane monooxygenase subunit A | 26,255 | 5597 | 0.21 |
7 | AYM39_11910 | Protein | Hypothetical protein | 17,722 | 48 | 0.00 |
8 | AYM39_15615 | Protein | Methanol dehydrogenase | 13,706 | 4962 | 0.36 |
9 | AYM39_15600 | Protein | Methanol dehydrogenase | 11,638 | 6170 | 0.53 |
10 | AYM39_02740 | Protein | 3-hexulose-6-phosphate synthase | 8076 | 2515 | 0.31 |
11 | AYM39_13140 | Protein | hypothetical protein | 6170 | 3378 | 0.55 |
12 | AYM39_08555 | Protein | formaldehyde-activating enzyme | 6016 | 8798 | 1.46 |
13 | AYM39_18825 | Protein | Cold-shock protein | 5653 | 6162 | 1.09 |
14 | AYM39_01615 | Protein | Hypothetical protein | 5370 | 1018 | 0.19 |
15 | AYM39_15605 | Protein | Cytochrome c(L), periplasmic | 5200 | 3475 | 0.67 |
16 | AYM39_02470 | Protein | 3-hexulose-6-phosphate synthase | 5164 | 1902 | 0.37 |
17 | AYM39_02670 | Protein | Coenzyme PQQ precursor peptide PqqA | 5132 | 3324 | 0.65 |
18 | AYM39_15610 | Protein | Methanol oxidation system protein MoxJ | 4917 | 2890 | 0.59 |
19 | AYM39_02475 | Protein | 6-phospho-3-hexuloisomerase | 4729 | 1926 | 0.41 |
20 | AYM39_02725 | Protein | Hypothetical protein | 3871 | 2311 | 0.60 |
* TPM: Transcript per Million. ** Fold values represent ratio between TPM values of samples cultured under 3.5% and 15% O2 conditions. Genes showing relatively constant expression in response to oxygen composition change (fold: around 1.0) are indicated in bold letters